Children as Research Collaborators: Issues and Reflections from a Mobility Study in Sub-Saharan Africa

This paper reflects on issues raised by work with children in an ongoing child mobility study in three sub-Saharan African countries: Ghana, Malawi and South Africa. There are now 70 school pupils of varying ages involved in the project, but the paper is particularly concerned with the participation of those children 14 years and under. We examine the significant ethical issues associated with working with younger child researchers, and linked questions concerning the spaces open to them in African contexts where local cultural constructions of childhood and associated economic imperatives (which commonly drive family and household endeavour) help shape the attitudes of adults to children’s rights and responsibilities and inter-generational power relations

The impact of ageing reveals distinct roles for human dentate gyrus and CA3 in pattern separation and object recognition memory

© 2017 The Author(s). Both recognition of familiar objects and pattern separation, a process that orthogonalises overlapping events, are critical for effective memory. Evidence is emerging that human pattern separation requires dentate gyrus. Dentate gyrus is intimately connected to CA3 where, in animals, an autoassociative network enables recall of complete memories to underpin object/event recognition. Despite huge motivation to treat age-related human memory disorders, interaction between human CA3 and dentate subfields is difficult to investigate due to small size and proximity. We tested the hypothesis that human dentate gyrus is critical for pattern separation, whereas, CA3 underpins identical object recognition. Using 3 T MR hippocampal subfield volumetry combined with a behavioural pattern separation task, we demonstrate that dentate gyrus volume predicts accuracy and response time during behavioural pattern separation whereas CA3 predicts performance in object recognition memory. Critically, human dentate gyrus volume decreases with age whereas CA3 volume is age-independent. Further, decreased dentate gyrus volume, and no other subfield volume, mediates adverse effects of aging on memory. Thus, we demonstrate distinct roles for CA3 and dentate gyrus in human memory and uncover the variegated effects of human ageing across hippocampal regions. Accurate pinpointing of focal memory-related deficits will allow future targeted treatment for memory loss

Een gekleurd reagens op SH groepen : toepassing bij de bepaling van de aminozuurvolgorde rondom de cysteinerest in serumalbuminen

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Send THE JOURNAL OF PHARMACOI.OGY AND EXPERIMENTAL THERAPEUTICS CORRELATION BETWEEN STRUCTURE, BEHAVIORAL ACTIVITY AND RATE OF BIOTRANSFORMATION OF SOME ACTH4-9 ANALOGS

ABSTRACT WIrrER, ALBERT, HEN

HLA-DQB1*0319, a novel HLA-DQB1 allele, shows strong haplotype association to HLA-DRB1*1102.

The new human leukocyte antigen-DQB1*0319 allele was identified in a prospective bone marrow donor by sequence-based typing. This novel allele differs from the DQB1*0301 allele at nucleotide position 554 (C-->T), which results in a Thr to Ile amino acid exchange. The new allele shows a strong association to DRB1*1102, suggesting that the haplotype DRB1*1102-DQB1*0319 is quite common

Sequence-based typing identifies a novel HLA-DPB1 allele, DPB1*9601

In this report we describe the identification of a novel HLA-DPB1 allele, DPB1*9601, found in a Caucasian individual sample named ucla#356. The new allele was detected in the DNA of ucla#356 during routine HLA sequence-based typing (SBT) of samples participating in the UCLA International HLA DNA Exchange (number 55) for HLA DNA Proficiency Testing. DPB1*9601 was identical to DPB1*3901 except for a single nucleotide substitution 'G'-->'C' in previously constant position 277 (position 177, respectively, counting only exon 2). This nucleotide change causes an amino acid substitution from aspartic acid in DPB1*3901 to histidine at codon 64 in the novel allele. This new allele has been submitted to the EMBL database and has been assigned the accession number AJ514871. The WHO Nomenclature Committee has officially assigned the name DPB1*9601